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Fig. 6 | Journal of Biomedical Science

Fig. 6

From: PES1 reduces CD8+ T cell infiltration and immunotherapy sensitivity via interrupting ILF3-IL15 complex in esophageal squamous cell carcinoma

Fig. 6

PES1 disrupts the interaction between ILF3 and IL15 mRNA. A–C IL15 mRNA stability was determined in PES1-knockdown KYSE150 cells (A) and AKR cells B and PES1-overexpressing EC9706 cells C treated with the transcription inhibitor actinomycin D for the indicated times (n = 3 independent experiments). **p < 0.01; ***p < 0.001. (D-E) RNA immunoprecipitation analysis of the binding of endogenous ILF3 and IL15 mRNA by ILF3 in KYSE150 (D) and EC9706 E cells (n = 3 independent experiments). F–G PES1 inhibits ILF3-IL15 mRNA interaction in EC9706 (F) and KYSE150 (G) cells by RNA immunoprecipitation with ILF3 antibody (n = 3 independent experiments). **p < 0.01; ***p < 0.001. H Working model showing that PES1 interferences the interaction between ILF3 and IL15 mRNA

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Journal of Biomedical Science

ISSN: 1423-0127