Fig. 3
Subsets of T cells, MBCs, monocytes and NK cells associated with progression to different dengue fever outcomes. A–H. Unsupervised identification of differentially abundant cell populations between DF (n = 6) or DHF (n = 6) clinical groups was performed using CITRUS (FDR < 1%). Differentially abundant populations were identified among: (A) TH1 memory CD4+ T cells; (B) TH2 memory CD4+ T cells; (C) TH17 memory CD4+ T cells; (D) circulating memory TFH cells; (E) memory CD8+ T cells; (F) MBCs; (G) monocytes; (H) NK cells. The tSNE plots in the top of each panel display cell density and represent pooled data for each clinical group as calculated in the clustering analysis shown in Fig. 2A-G, while the middle panels show differentially abundant populations identified in colours on a tSNE overlay, and the viSNE plots on the left-hand side from each top panel depict relevant marker expression on tSNE overlays. The lower left panels show the frequency of differentially abundant cell populations identified by CITRUS. Boxes represent the 25th to 75th percentiles, whiskers show the range (minimum to maximum), and lines represent the median of 6 (DF) and 6 (DHF) biological replicates. The lower right panels illustrate marker expression in differentially abundant populations (pink histograms), relative to background expression (lilac histograms)