Fig. 3

PUM2 negatively regulates mRNA expression of DLX5. A qPCR data using RNA from MSCs transfected with siRNA targeting PUM2, compared to the group transfected with negative control (NC) siRNAs (n = 3 experimental replicates). B PUM2 and DLX5 protein levels were analyzed by western blotting for MSCs transfected with NC or PUM2 siRNA. C PUM2::GFP and DLX5 protein levels were analyzed in MSCs using western blotting for cells transfected with the pEGFP-C1 vector control or the pEGFP-C1-PUM2 vector. D PUM2::GFP-immunoprecipitation was successful; the bar graph displays the RIP-qPCR fold enrichment of mRNAs relative to the IgG control. *P < 0.05 in a comparison with the IgG control (n = 3 experimental replicates). E GFP and DLX5 levels were analyzed in MSCs using western blotting after transfection with the pEGFP-C1 vector control, pEGFP-C1-PUM1, or pEGFP-C1-PUM1 mutant vector (PUM2-ΔHD)