Fig. 5

PROTAC 2 reduced C-TDP-43 aggregation and improved the motility of the neuronal YFP-C-TDP-43 transgenic C. elegans. A, B Schematic drawings of neuronally expressing C. elegans and its ventral cord. C, D Illustration of YFP (C) and YFP-C-TDP-43 (D)expression pattern within the region of interest in panel B. E Representative images of either YFP control or YFP-C-TDP-43 transgenic C. elegans with or without PROTAC 2 (5 μM) or/and MG132 (5 μM). While the cytosolic aggregates within the ventral cord were visualized in YFP channel (green), the nuclei of neuron cell bodies were monitored in DAPI channel (pseudo red color). Scale bar = 10 μm. F The relative YFP intensity of YFP-C-TDP-43 strain in panel E. G The bending frequency of YFP-C-TDP-43 transgenic C. elegans with or without PROTAC 2 (5 μM) or/and MG132 (5 μM) and YFP control. Each dot represents an independent experiment containing at least 15 worms with three repeat videos. All the statistic results were quantified by ImageJ and shown as mean ± SD (n ≥ 3). Data were analyzed by one-way ANOVA with Tukey post-hoc test (*P < 0.05, ***P < 0.001)