Fig. 1

Acetaldehyde sustains ethanol (EtOH)-evoked periorbital mechanical allodynia (PMA). a Dose-dependent motor coordination and b dose- and time-dependent PMA evoked by EtOH (intragastric, i.g.) or vehicle (veh, 0.9% NaCl) in C57BL/6J mice. c PMA evoked by EtOH (15%, 4 ml/kg, i.g.) or veh in C57BL/J mice pretreated (30 min before) with capsazepine (CPZ, 4 mg/kg, i.p.) or veh. d PMA evoked by EtOH (15%, 4 ml/kg, i.g.) or veh in Trpv1^+/+ and Trpv1^−/− mice. e, f PMA evoked by EtOH (15%, 4 ml/kg, i.g.) or veh in C57BL/6J mice pretreated (30 min before) with 4-methylpyrazole (4-M, 50 mg/kg, i.g. or 100 nmol, periorbital, p.orb.), or veh (n = 8 mice for behavioral experiments). g Acetaldehyde (ACD) levels in the infraorbital trunk of the trigeminal nerve of C57BL/6J mice receiving EtOH (15%, 4 ml/kg, i.g.) (n = 4 mice for each condition). h ACD levels in the infraorbital trunk of the trigeminal nerve of C57BL/6J mice 3 h after EtOH (15%, 4 ml/kg, i.g.) or veh and pretreated (30 min before) with 4-M (50 mg/kg, i.g. or 100 nmol, p.orb.) (n = 4 mice for each condition). Dash (-) is the combination of veh. *P < 0.05 vs. veh, time 0; ^#P < 0.05 vs. 4-Mp-EtOH, one and two-way ANOVA with Bonferroni post-hoc correction