Fig. 2

TRPA1 mediates periorbital mechanical allodynia (PMA) evoked by ethanol (EtOH) and acetaldehyde (ACD). a Dose- and time-dependent PMA evoked by ACD (i.p.) or vehicle (veh, 0.9% NaCl) in C57BL/6 J mice. b, c PMA evoked by ACD (0.1 mg/kg, i.p.), EtOH (15%, 4 ml/kg, i.g.), or veh in Trpa1^+/+ and Trpa1^−/− mice. d, e PMA evoked by ACD (0.1 mg/kg, i.p.), EtOH (15%, 4 ml/kg, i.g.), or veh in C57BL/6J mice treated (2 h after) with A967079 (A96 300 nmol, periorbital, p.orb.) or veh. (f, g) PMA evoked by ACD (0.1 mg/kg, i.p.), EtOH (15%, 4 ml/kg, i.g.), or veh in C57BL/6J mice treated (2 h after) with A96 (100 mg/kg, i.p.) or veh. (n = 8 mice). *P < 0.05 vs. veh; ^#P < 0.05 vs. Trpa1^+/+-EtOH, Trpa1^+/+-ACD, veh A96-EtOH, ACD, two-way ANOVA with Bonferroni post-hoc correction