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Fig. 4 | Journal of Biomedical Science

Fig. 4

From: Acetaldehyde via CGRP receptor and TRPA1 in Schwann cells mediates ethanol-evoked periorbital mechanical allodynia in mice: relevance for migraine

Fig. 4

Schwann cell TRPA1 receptor activation mediates periorbital mechanical allodynia (PMA) evoked by ethanol (EtOH) and acetaldehyde (ACD). a, b Time-dependent PMA evoked by EtOH (15%, 4 ml/kg, i.g.), ACD (0.1 mg/kg, i.p.), or vehicle (veh, 0.9% NaCl) in Plp1-CreERT+;Trpa1fl/fl and Plp1-CreERT−; Trpa1fl/fl (control) mice. c, d H2O2 levels in the infraorbital trunk of the trigeminal nerve of C57BL/6J mice receiving EtOH (15%, 4 ml/kg, i.g.), ACD (0.1 mg/kg, i.p.) or veh. e Representative photomicrographs and mean fluorescence intensity of 4-hydroxy-nonenal (4-HNE) staining in the infraorbital trunk of the trigeminal nerve of C57BL/6J mice receiving EtOH (15%, 4 ml/kg, i.g.), ACD (0.1 mg/kg, i.p.), or veh (0.9% NaCl) (scale bar: 10 µm) (n = 4 mice for each condition). PMA evoked by f, g EtOH (15%, 4 ml/kg, i.g.), h, i ACD (0.1 mg/kg, i.p.) or veh in C57BL/6J mice treated (2 h after) with PBN (100 mg/kg, i.p. or 670 nmol, periorbital, p.orb.) or veh. j, k PMA evoked by EtOH (15%, 4 ml/kg, i.g.), ACD (0.1 mg/kg, i.p.), or veh in Adv-Cre+;Trpa1fl/fl and Adv-Cre−;Trpa1fl/fl (control) mice. (n = 8 mice). *P < 0.05 vs. veh, time 0; #P < 0.05 vs. control -EtOH, ACD, Adv-Cre−;Trpa1fl/fl -EtOH, ACD, veh PBN-EtOh, ACD

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