Fig. 1

The dynamic changes of eIF2 O-GlcNAcylation upon ArgS. A A Venn diagram was generated to depict the overlap between 2054 O-GlcNAz-modified proteins that were pulled down (Additional file 3: Table S4, S5) in different ArgS-induced pathways (as obtained from Reactome.org). B A pathway enrichment analysis was performed on O-GlcNAz-modified proteins (using the WIKIPATHWAYS database) to identify those significantly affected (FDR < 0.05) by ArgS. C The 363 O-GlcNAz-modified proteins identified in the pathway enrichment analysis B were grouped by function using the STRING database. D A heatmap was generated to show the log10 abundance ratio of O-GlcNAz-modified proteins involved in eukaryotic protein translation that were significantly changed (FDR < 0.05) based on proteomics analysis under the indicated conditions. The O-GlcNAz-modified eukaryotic factors/regulators were clustered based on their level changes relative to control, with samples prepared from BT-549 cells (n = 3). The cells were treated with an -Arg medium (or not) and GlcNAz (50 μM) for 48 h prior to collection and protein extraction