Figure 4
Effect of PHGG on 30% FeCl 3 -induced 4-HNE, ICAM-1 and TTO in the injuried carotid artery of the hamsters with HF diet. Oxidative stress indicated by 4-HNE (A-D) and ICAM-1 (E-H) immunostaining of hamster carotid arterial sections subjected to 150 sec of FeCl3-induced arterial injury. Significantly enhanced endothelial ICAM-1 (brown color in B) and and 4-HNE (brown color in F) are found in HF group subjected to FeCl3 treated carotid artery. The amplified diagram in the right corner was displayed. Carotid arterial 4-HNE and ICAM-1 expression were significantly reduced in HFLP and HFHP groups. FeCl3 induced time to occlusion (TTO) of carotid arteries obtained from control (n = 11), HF (n = 11), HFLP (n = 11) and HFHP (n = 11) groups is indicated in the I. HF shortened TTO, but was reversed by PHGG treatment. *P < 0.05 vs. control group. # P < 0.05 vs. HF group.