| HSA (102 × dpm) |
---|
 | Control | Simvastatin (1 μM) | Simvastatin (5 μM) |
---|
Intra peak * | 350 ± 31 | 414 ± 24 | 403 ± 52 |
Depletion | 216 | 237 | 243 |
Secretion | 168 | 173 | 143 |
Degradation | 48 | 64 | 100 |
% Recovery ** | 86.3 | 84.5 | 75.2 |
- Cells were incubated for 22 hours with 0, 1, and 5 μM simvastatin, then cell were pulse-labeled for 20 minutes with [35S] methionine. After pulse-labeling, media were washed and incubated for 20, 120 minutes in media containing an excess of unlabeled methionine/cysteine. At the end of each chase period, solubilized cell lysates and media were subjected to immunoprecipitation. Quantification of labeled HSA was determined by SDS-PAGE and scintillation counting. Data presented are from three separate experiments done in duplicate.
- * Intra peaks were calculated from the HSA content at 20 minutes of chase
- ** % recovery represents total labeled apoB (cell lysate + media) remained after 2-hour chasing.