Figure 3

Tracings showing [Ca²⁺] _i (indicated by fluorescence ratio of fura-2 at 340 nm and 380 nm) transient in rat aortic endothelial cells. ACh (3 μmol/L) elicited increases in [Ca²⁺]_i in fura-2-loaded endothelial cells. Vehicles of LPC and benidipine had no effect on ACh-induced increases in [Ca²⁺]_i (A). LPC (3 μmol/L) decreased ACh-induced increases in [Ca²⁺]_i (B). Benidipine (10 nmol/L) prevented LPC-induced suppression of increases in [Ca²⁺]_i (C).