Figure 1
From: Formulation of novel lipid-coated magnetic nanoparticles as the probe for in vivo imaging
Magnet effect on L-SPIOs loading in HeLa cells. (A) Size distribution of L-SPIOs particles. (B) Images of HeLa cells treated with 1 nmole/well of L-SPIOs for 2 h in 10% FBS-DMEM with magnet (right panel) or without magnet (left panel). Trace amount of Rhodamine-DOPE was used as an indicator for localizing the position of the nanoparticles. Images were taken after L-SPIO treatment (phase and fluorescent images: 100× magnification; Prussian blue staining: 400× magnification). (C) Time-dependent effect of magnet on cell loading. HeLa cells were seeded overnight and treated with 1 nmole/well of L-SPIOs for 0, 0.5, 1 and 2 h. These fluorescent images were taken at different time points as indicated on the right. (D) Flowcytometric analysis of cells from (C) was shown. (E) Confocal image of L-SPIO-loaded HeLa cells. HeLa cells treated with L-SPIOs (1 nmole/well) for 16 h. After washed with cold PBS, cells were fixed and analyzed by a confocal microscope. Red fluorescence in cells was indicated as L-SPIOs, whereas blue fluorescence was nucleus. Scale bar = 40 μm. Data represent three independent experiments with similar results.