Figure 1
From: Immobilizing topoisomerase I on a surface plasmon resonance biosensor chip to screen for inhibitors
Purification of recombinant human topoisomerase I (hTopI) obtained using a baculovirus expression system (lane 1, cell lysate; lane 2, partial purified fraction; and lane 3, Ni-NTA column purified protein) (left panel). Purified hTopI was further verified by Western blot analyses using serially diluted protein amounts (20, 10, and 5 μg/lane), and probed with rabbit antibodies against hTopI (right).