Figure 2

Preferential transcriptional upregulation of HO-1 by HIF-1 in RVLM during the pro-life phase. A. Illustrative gels or summary of fold changes against aCSF controls in ratio of HO-1 or HO-2 relative to β-actin protein detected in ventrolateral medulla of rats that received immunoneutralization of HIF-1α, HIF-1β or HIF-2α subunit in RVLM, 1 h before induction of Mev intoxication. B. Illustrative gels or summary of fold changes against aCSF controls of HO-1 expression detected in ventrolateral medulla of rats that received immunoneutralization of SUMO-1 or Ubc9, 1 h before induction of Mev intoxication. Values in A and B are mean ± SEM of triplicate analyses on samples pooled from 4-6 animals per experimental group. *P < 0.05 versus aCSF group and ⁺P < 0.05 versus Mev group in the Scheffé multiple-range test. Note that dividing lines are placed on the gel images to denote groupings of images from different parts of the same gel. Note also that numbers on top of the gels in B correspond to columns in the data summary.