Figure 3From: Direct electrochemical analyses of human cytochromes b5 with a mutated heme pocket showed a good correlation between their midpoint and half wave potentialsCyclic voltammogram of HLMW b 5 in 50 mM sodium phosphate buffer pH 7.0. (Panel A) The gold electrode was modified with β-mercaptopropionic acid and the voltammogram of HLMWb5 (100 μM (final) in 50 mM sodium phosphate buffer pH 7.0) was obtained in the presence of 200 μM of poly-L-lysine. The potential shown is vs. an Ag/AgCl reference electrode with an internal filling solution of 3 M KCl saturated with AgCl (E° = +197 mV vs. SHE). Scan rate = 100 mV/sec. (Panel B) Plot of the anodic peak current Ipa against the square root of the scan rate ν1/2.Back to article page