Figure 4

The effect of SBG on LPS induced expression of COX-2 and iNOS protein with the Raw 264.7 cells and BV2 cells. The Raw 264.7 cells (A) and BV2 cells (B) were pretreated with the indicated concentrations of SBG (SBG100, 100 ug/ml, SBG200, 200 ug/ml) for 30 min before incubation with LPS for 12 h. The cells were lysed and the lysates were analyzed by immunoblotting using anti COX-2, iNOS. Values are expressed as mean ± standard deviation (mean ± S.D.), and analyzed by one-way ANOVA and multiple comparisons were performed by Tukey HSD test. A criterion of significance was accepted and marked as P < 0.001 (###). The mark of star is AC group versus NC group and the mark of pound is each treatment group versus AC group.