Figure 6

The effect of SBG on the caspase-dependent apoptosis molecules in Raw 264.7 and BV2 cells. The Raw 264.7 (A) and BV2 cells (B) were pretreated with the indicated concentrations of SBG (100 ug/ml and 200 ug/ml) for 30 min before incubation with LPS for 12 h. The cells were lysed and the lysates were analyzed by immunoblotting using anti cleaved caspase-3, bcl-2. Values are expressed as mean ± standard deviation (mean ± S.D.), and analyzed by one-way ANOVA and multiple comparisons were performed by Tukey HSD test. A criterion of significance was accepted and marked as P < 0.01 (##) and 0.001 (###). The mark of star is AC group versus NC group and the mark of pound is each treatment group versus AC group.