Figure 4From: A naturally occurring carotenoid, lutein, reduces PDGF and H2O2 signaling and compromises migration in cultured vascular smooth muscle cellsLutein affects cellular components. (A) Cells were incubated with vehicle or lutein (10 μM) for 16 h and cell viability was measured by MTT assay as described in Methods. (B) Cells were preincubated with vehicle or lutein (10 μM) for 1 or 6 h and followed by an extensive wash to remove extracellular unbound lutein. Cells were then stimulated with PBS or PDGF for 10 min. PDGFR and PLCγ phosphorylation was examined by Western blotting and quantitation was performed by densitometry (n = 3).Back to article page