Figure 3

ssh , but not tsr , share a similar phenotype to capt. (A-C) ssh^1-63 mutant phenotype. (A) ssh^1-63 mutant MARCM clones (GFP, blue) labeled for Arm (green). (B) Large ssh^1-63 mutant MARCM clones labeled for p-MLC (red) and actin (green). (C) Strong accumulation of F-actin (red) was detected only in ssh^1-63 mutant MARCM clones (GFP, blue) posterior but not anterior (asterisk) to the MF. (D-F) tsr^N96Amutant phenotype. (D,E) tsr^N96A mutant MARCM clones (GFP, blue) within the MF labeled for F-actin (red in D), p-MLC (red in E) and Arm (green). (F) tsr^N96A mutant MARCM clones (GFP, blue) posterior to the MF labeled for F-actin (red) and Arm (green). Arrowheads indicate tsr^N96A mutant ICs with elevated F-actin. (G) No excessive F-actin (red) accumulation, hexagonal cell shape or discontinuous AJs (Arm, green) were detected in twf¹¹⁰ mutant MARCM clones (GFP, blue) within and posterior to the MF. In all images, lines indicate the position of the MF, and anterior is to the top. Bars, 2 μm.