Figure 6From: Natural diterpenes from coffee, cafestol and kahweol induce apoptosis through regulation of specificity protein 1 expression in human malignant pleural mesotheliomaThe effect of cafestol and kahweol on apoptosis of MSTO-211H cells. Fifty micrograms of cellular extract per lane were separated on an SDS-PAGE gel as described in materials and methods. Immunoblot detection of Bid, Caspase-3, and PARP, upregulation of Bax, downregulation of Bcl-xl in whole cell lysates from various concentration of cafestol and kahweol-treated MSTO-211H cells for 48 hours. Equal loading of protein was confirmed by incubating the same membrane with anti-β-Actin antibody.Back to article page