Figure 2

CLSM images of apoptosis and/or necrosis in human squamous cell carcinoma (COLO-16) cell line. Cells were incubated for 3 h with 100 ng/mL, 200 ng/mL or 400 ng/mL of RRM-MV or RRM-C. After incubation, cells were double stained with AF488 and PI. These CLSM images show that RRM-MV induced cell apoptosis/necrosis effects. AF488 channels show apoptotic cells labeled with green-fluorescent and PI channels show necrotic cells labeled with red-fluorescent (100x magnification).