Figure 6
Effect of caspase-8 inhibitor and caspase-9 inhibitor on the apoptosis induced by PSE in AGS gastric cancer cells. (A) AGS Cells were seeded in 12-well culture plates at a density of 5 × 104 cells/well. The next day, cells were treated with caspase-8 inhibitor (40 □M) or caspase-9 inhibitor (40 □M) in the presence or absence of 200 μg/ml PSE for 3 days. Cells were harvested by trypsinization, resuspended in 1–2 ml of medium, and counted using a hemocytometer. Bars, SD. *p < 0.05, ***p < 0.001. (B) AGS cells were exposed to 200 μg/ml PSE and 0.1% DMSO either with or without caspase-8 inhibitor (40 □M) and caspase-9 inhibitor (40 □M) for 24 h, the cell lysates were separated by SDS-PAGE gel electrophoresis, and Western blotting with specific antibodies was performed (anti-cleaved caspase-8, anti-cleaved caspase-3, anti-cleaved-PARP, and anti-Tubulin). The data shown are representative of three independent experiments that gave similar results.