Figure 3From: Houttuynia cordata Thunb extract modulates G0/G1 arrest and Fas/CD95-mediated death receptor apoptotic cell death in human lung cancer A549 cellsEffects of HCT on DNA condensation, damage and apoptosis in human lung cancer A549 cells. (A) A549 cells stained with DAPI to observe DNA condensation after 24 h of treatment with 500 μg/ml of HCT. (B) A549 cells were examined DNA damage by Comet assay after 24 h of treatment with 500 μg/ml of HCT. Cells were photographed under fluoresce microscopy (x200) as described in Materials and Methods. (C) A549 cells were treated with HCT (0, 125, 250 and 500 μg/ml) for 24 h, and then were harvested for determination the sub-G1 (apoptosis) cell population by flow cytometry as described in Materials and Methods. (D) The bar diagram of the length of Comet tail. The experiments were performed in triplicate (n = 3). *p < 0.05, ***p < 0.001 was considered significantly different in comparison with the control.Back to article page