Table 2 HAI antibody titer of mice post immunized with S-OIV H1N1 vaccine in the absence or presence of adjuvant
Adjuvant | Sample dose | 1 | 2 | 3 | 4 | 5 | 6 | 7 | HAI ≥40 | HAI titer | Mean of HAI titer |
|---|---|---|---|---|---|---|---|---|---|---|---|
Pre-immune | – | – | – | – | – | – | – | 0/7 | 0 | 0 | |
Non adjuvant | PBS | – | – | – | – | – | 0/5 | 0 | 0 | ||
0.05 μg | 80 | 160 | – | – | – | 80 | 160 | 4/7 | 80 ~ 160 | 68.6 | |
0.01 μg | 160 | – | – | – | – | 40 | – | 2/7 | 40 ~ 160 | 28.6 | |
0.001 μg | – | – | – | 40 | – | * | – | 1/6 | 40 | 6.7 | |
Alum | PBS | – | – | – | – | – | – | – | 0/6 | 0 | 0 |
0.05 μg | 640 | 1280 | 80 | 640 | 1280 | 80 | 160 | 7/7 | 80 ~ 1280 | 594.3 | |
0.01 μg | 160 | 40 | 80 | 160 | 160 | 80 | 320 | 7/7 | 80 ~ 320 | 142.9 | |
0.001 μg | 80 | – | – | – | 40 | 80 | 80 | 4/7 | 40 ~ 80 | 40.0 | |
MPLA | PBS | – | – | – | – | – | – | – | 0/6 | 0 | 0 |
0.05 μg | 160 | 320 | 320 | – | 160 | 160 | 640 | 6/7 | 160 ~ 640 | 251.4 | |
0.01 μg | 320 | 80 | 160 | – | 160 | 80 | 80 | 6/7 | 80 ~ 320 | 125.7 | |
0.001 μg | 80 | – | – | – | * | 80 | 40 | 3/6 | 40 ~ 80 | 33.3 |