Figure 7

H-rev107 suppresses Rac1 activation and increases E-cadherin expression. NT2/D1 cells grown to 80% confluence were transfected with the indicated control or H-rev107 expression vector and then incubated with 500 ng/mL of PGD2 or ethanol vehicle for 24 h. Cells were serum starved for 12 h and then stimulated by EGF (50 ng/mL) for 5 min. Cellular lysates were incubated with agarose conjugated with PAK-1 PBD. After washing, the bound activated Rac1 (Rac1-GTP) was analyzed by Western blotting (A). NT2/D1 cells were transfected with H-rev107 or control expression vector and then incubated with 500 ng/mL of PGD2 or ethanol vehicle for 24 h. The levels of E-cadherin and vimentin were determined by Western blot analysis (B).