Figure 2

MiR-488 is involved in degeneration of human articular chondrocytes. (A) Human articular chondrocytes isolated from biopsy normal cartilage were treated with 10 ng/ml IL-1β in the presence of miR-488 or miR-488 inhibitor (anti-miR-488), stained with phalloidin (left panel), and expression level of type II collagen was analyzed (right panel). (B) Human articular chondrocytes isolated from biopsy normal cartilage were treated with 10 ng/ml IL-1β or 5 ng/ml TGF-β3, stained with phalloidin (left panel), and expression level of type II collagen and miR-488 were analyzed by real-time PCR (right panel). *, statistically different from control cells (p < 0.001). The error bars represent average of data from each human sample.