Figure 9
From: Involvement of STIM1 and Orai1 in EGF-mediated cell growth in retinal pigment epithelial cells
Involvement of Orai1 and STIM1 in EGF-mediated cell proliferation and migration. (A i, ii) ARPE-19 cells were transfected with another pair of Orai1 and STIM1 siRNA. Expression of Orai1 and STIM1 was detected by real-time PCR (i) and Western blots (ii). (B-C) ARPE-19 cells were transfected with Orai1 and STIM1 siRNA. WST-1 assay or cell migration assay was used. (D) Quantitative analysis of ARPE-19 cell migration of Figure 9C. (E) ARPE-19 cells were transfected with Orai1 and STIM1 siRNA. EGF-mediated phosphorylation of ERK 1/2 and Akt were detected by Western blots.