Figure 4From: Hes-1 SUMOylation by protein inhibitor of activated STAT1 enhances the suppressing effect of Hes-1 on GADD45α expression to increase cell survivalIdentification of candidate SUMO sites on Hes-1. Flag-Hes-1WT plasmid or individual Flag-Hes-1 lysine residue mutant plasmid was co-transfected with Myc-SUMO-1 and EGFP-PIAS1 to HK293T cells and the cell lysate was subject to western blot against various tags. (A) Gel pattern showing Hes-1 SUMOylation upon transfection of Hes-1 lysine residue mutant from Lys8 (K8R) to Lys86 (K86R). The quantification result is shown in the lower panel. (B) Gel pattern showing Hes-1 SUMOylation upon transfection of Hes-1 lysine residue mutant from Lys109 (K109R) to Lys215 (K215R). Lysine residue mutants from Lys8 to Lys58 were repeated for the purpose of comparison. The quantification result is shown in the lower panel. Results were obtained from two independent experiments. The sumoylated Hes-1 bands are shown in the bracket. Data are expressed as mean ± SEM (standard error of mean). * p < 0.05, ** p < 0.01 and # p < 0.001.Back to article page