Hes-1 SUMOylation by protein inhibitor of activated STAT1 enhances the suppressing effect of Hes-1 on GADD45α expression to increase cell survival

Table 1 Primers for plasmid construction

Pias1	Forward: 5’-ATCGGGATCCCATGGCGGACAGTGCGGAAC-3’
	Reverse: 5’-ATCGGAATTCTCAGTCCAACGAGATAATG-3’
Pias2	Forward: 5’-ATCGGAATTCGATGGCGGATTTCGAGGAG-3’
	Reverse: 5’-ATCGCTCGAGTCACTGTTGCACAGTATC-3’
Pias3	Forward: 5’-ATCGGAATTCGATGGCGGAGCTGGGCG-3’
	Reverse: 5’-ATCGCTCGAGTCAGTCCAAGGAAATG-3’
Pias4	Forward: 5’-ATCGGAATTCGATGGCGGCAGAGCTGGTG-3’
	Reverse: 5’-ATCGCTCGAGTCAGCAAGCGGGCACCAG-3’
Hes-1	Forward: 5’-ATGCCAGCTGATATAATGG-3
	Reverse: 5’-TCAGTTCCGCCACGGCCTC-3
Hes-5	Forward: 5’-GACTGGATCCATGGCCCCAAGTACCGTGG-3’
	Reverse: 5’-GACTGAATTCTCACCAGGGCCGCCAGAG-3’
RanBP2	Forward: 5’-ATGCGAATTCTTGAAAAGTAACAATAG-3’
	Reverse: 5’-ATGCCTCGAGAACTATCTTGCTTTCC-3’
Pc2	Forward: 5’-ATCGGAATTCGATGGAGCTGCCAGCTGTTG-3’
	Reverse: 5’-ATCGCTCGAGCTACACCGTCACGTATTC-3’
SUMO1	Forward: 5’-GCAACCCGGGTGTCTGACCAGGAGGCAAAACCTTC-3’
	Reverse: 5’-GCAAGGTACCCTAAACCGTCGAGTGACCCCCCGT-3’
Ubiquitin	Forward: 5’-CCATGGATGCAGATCTTCGTGAAGAC-3’
	Reverse: 5’-GGATCCTTAGACACCCCCCCTCAAGC-3’
GADD45α	Forward: 5’-GACTGAGCTCCTTAGGGCATATCGAGAGCAT-3’
	Reverse: 5’-GATCAGATCTAAAGTCATATTGCAAACTGCAGGTC-3’

ISSN: 1423-0127