Table 1 Primers used for real time quantitative PCR to detect gene expression in H9c2 cells
Accession no. | mRNA name | Primer sequence (5’ → 3’) | Size of products (bp) |
|---|---|---|---|
NM_032416.1 | Aldehyde dehydrogenase 2 family (mitochondrial) (Aldh2) | Forward: TGGCTGATCTCATCGAACGG (360–379) | 134 |
Reverse: CCAGCCAGCATAATAGCGGA (493–474) | |||
NM_012554.3 | Enolase 1, (alpha) (Eno1) | Forward: CCTACTGCCAGAACTTCACCA (102–122) | 208 |
Reverse: GAGACACCCTTCCCCATGAA (309–290) | |||
NM_057141.1 | Heterogeneous nuclear ribonucleoprotein K (Hnrnpk) | Forward: CACCTTGCTTTGTGGTCACTG (1700–1720) | 232 |
Reverse: TTAGTTTAGGGGTGGGCTGG (1931–1912) | |||
NM_001007149.1 | Staufen, RNA binding protein, homolog 2 (Drosophila) (Stau2) | Forward: CAGAGCGGGGTCATTTCTCG (25–44) | 220 |
Reverse: GGATGCTATGGAAACGGGCT (244–225) | |||
NM_022521.3 | Ornithine aminotransferase (Oat) | Forward: CAGGGTGAAGCGGGTGTTAT (803–822) | 262 |
Reverse: CGTGCTCGCCTGGTTTAATG (1064–1045) | |||
NM_053917.1 | Inositol polyphosphate-4-phosphatase, type II (Inpp4b) | Forward: ATGGAAAAGATGCCGCCTGA (2739–2758) | 239 |
Reverse: TCGTCTCTCAGGATGGAGCA (2977–2958) | |||
NM_053512.2 | Peroxiredoxin 4 (Prdx4) | Forward: GCCAAGATTTCCAAGCCAGC (268–287) | 284 |
Reverse: CTTATTGGCCCCAGTCCTCC (551–532) | |||
NM_130428.1 | Succinate dehydrogenase complex, subunit A, flavoprotein (Fp) (Sdha) | Forward: ATGGGCGAACCTACTTCAGC (793–812) | 84 |
|  |  | Reverse: AAGGTAAACCAGCCCGAGTG (876–857) |  |