Figure 4

The expression of miRNA-targeted genes in dMSCs from patients with PE and healthy pregnant women. A: The serum was isolated from five healthy pregnant women and five patients with PE, and concentration of VEGFA in serum was assessed using ELISA. B: The serum was isolated from five healthy pregnant women and five patients with PE, and concentration of IDO in serum was assessed using ELISA. C: dMSCs were isolated from five healthy pregnant women and five patients with PE as described in Materials and Methods. Then the expression of SOCS3 was detected by qPCR. D: dMSCs were isolated from five healthy pregnant women and five patients with PE as described in Materials and Methods. Then the expression of PPP2R2A was detected by qPCR. dMSC, decidua-derived mesenchymal stem cell; ELISA, enzyme-linked immunosorbent assay; IDO, indoleamine 2,3-dioxygenase; miRNA, microRNA; PE, pre-eclampsia; PPP2R2A, serine/threonine protein phosphatase 2A 55 kDa regulatory subunit B α isoform; qPCR, quantitative polymerase chain reaction; SOCS3, suppression of cytokine signaling 3; VEGFR, vascular endothelial growth factor.