Recombinant Lactobacillus plantarum expressing and secreting heterologous oxalate decarboxylase prevents renal calcium oxalate stone deposition in experimental rats

Table 1 Bacterial strains and plasmids used in this work

Strains & plasmids	Characteristics^α	Source/references
Strains
L. plantarum
WCFS1*	Host strain, Plasmid-free, silage isolate	Kleerebezem et al.,[19]
NC8OxdC	p256/pUC(pGEM)ori;P_ldhL;oxdC;Erm^r	Kolandasamy et al.,[17]
WCFS1OxdC	p256/pUC(pGEM)ori;P_ldhL;sp_{Lp_0373} fused to the oxdC; Erm^r	Sasikumar et al.,[16]
Plasmid
pLdhlOxdC	p256/pUC(pGEM)ori;P_ldhL;oxdC;Erm^r	Kolandasamy et al.,[17]
pLdhl0373OxdC	p256/pUC(pGEM)ori;P_ldhL;sp_{Lp_0373} fused to the oxdC; Erm^r	Sasikumar et al.,[16]

^αFor strains, genotypic and phenotypic characteristics are given; for plasmid, plasmid and cloned-cassette characteristics are given; Erm^r, : resistance to erythromycin.
*L. plantarum WCFS1 is a single colony isolate of strain NCIM8826 (Kleerebezem et al., 2003) [19].

ISSN: 1423-0127