Figure 4

Pretreatment with EPA or DHA prevents the doxorubicin-induced decrease in UCP2 protein expression. H9C2 cells were left untreated or were treated with 50 μM DHA or 100 μM EPA for 24 h, then were left untreated or wre treated with 1 μM doxorubicin (DOX) in the continued presence of the fatty acid for 24 h. After treatment, the cells were harvested and proteins extracted for Western blotting using antibody against UCP2 with ß-actin as the internal control. The bars are the quantitative density analysis expressed as the relative density compared to that in the untreated control and are the mean ± S.D. for three separate experiments. **, ***: p <001 and p <0.005 compared to the untreated control, respectively; ^#: p <0.05 compared to doxorubicin treatment.