Figure 1From: Effects of tobacco smoking during pregnancy on oxidative stress in the umbilical cord and mononuclear blood cells of neonatesReactive oxygen species (ROS) production. (A) Representative histograms from flow cytometry analysis using dihydroethidium (DHE) and 2',7'-dichlorofluorescein (DCF) in umbilical cord mononuclear blood cells from non-smokers and smokers. The log fluorescence (X-axis) illustrates the intensity of fluorescence for the number of cells counted. (B) Bar graph showing a remarkable increase in the level of superoxide anions (by DHE) and hydrogen peroxide (by DCF) in the smokers group (n = 23). The values are presented as means ± SEM. *p < 0.05 vs. non-smokers group (n = 30).Back to article page