Figure 4

GFP signal with Ad-CMV-GFP or Ad-PGK-GFP transduction in A549 and H1299 cells. (A) Real-time RT-PCR of mRNA level and (B) western blot analysis of protein level of coxsackie and adenovirus receptor (CAR) in H1299 and A549 cells. Fluorescence microscopy of GFP in (C) H1299 and A549 cells with Ad-CMV-GFP transduction at MOI 10 for 6 h and (D) A549 cells with Ad-CMV-GFP and Ad-PGK-GFP transduction at MOI 10 for 18 h. Data are mean ± SEM (n = 6). *P < 0.05 and ***P < 0.001.