Fig. 6

Intraperitoneal LPS infusion induces a COX-2-dependent microglial activation in the hippocampus. Gels (inset) and densitometric analysis of results from Western blot showing changes in the expression of Iba-1 (a) or representative photomicrographs (b-d) showing immunoreactivity to Iba-1 in hippocampal area (CA1, CA3, and dentate gyrus, DG), measured on day 7 after intraperitoneal infusion via an osmotic minipump of saline or LPS (2.5 mg/kg/day) for 7 days alone or with additional intracerebroventricular infusion of NS398 (5 μg/μl/h, dissolved in 1 % DMSO), tempol (2.5 μg/μl/h, dissolved in saline) or the corresponding vehicle. Also shown are photomicrographs of a typical activated microglia in a higher magnification (insets in b-d). Values are mean ± SEM, n = 8–10 rats per group. *P <0.05 vs. saline-treated group; ^#P <0.05 vs. LPS-treated group in the post hoc Scheffé multiple-range test. Scale bar in (b-d): 100 μm