Fig. 5

Mitochondrial Top3α deficiency leads to mitochondrial genome instability. a NGS analysis revealed elevated mtDNA deletions in 5-week-old M1L flies. b The frequency of DNA deletions in mtDNA genes. All values were normalized to those of 1-week-old wildtype. The lower panel is a schematic diagram showing the relative location of genes on mtDNA. Arrows above the line indicate genes that are encoded on the light strand, while arrows below the line indicate genes on the heavy strand. Protein- and RNA-coding genes are marked by orange and brown arrows, respectively. c Detailed features of the deletion hotspot surrounding the tRNA cluster (brown arrows with amino acids marking each tRNA species)