Fig. 3

YAP1 is a functional target of miR-199a-3p in HCC cells. a Bioinformatics-based target prediction analysis represents that YAP1 is a potential target of miR-199a-3p and the putative binding sequence is in the 3’-UTR of YAP1 mRNA. b Luciferase reporter assay shows that the luciferase activity in cells containing miR-199a-3p and 3’UTR-WT of YAP1 is markedly decreased compared with the negative control and 3’UTR-MUT groups. c and d qRT-PCR and western blot analyses shows that miR-199a-3p overexpression signigficantly downregulates mRNA and protein levels of YAP1.e–g MTT, flow cytometry and Caspase 3/7 activation assays show that the upregulation of YAP1 partially reverses miR-199a-3p’s effect on the proliferation and apoptosis of Huh7 cells. *P < 0.05, **P < 0.01 and ***P < 0.001