Fig. 3
From: Adducin family proteins possess different nuclear export potentials
ADD2 and ADD3 counteracted the effect of the NLS derived from SV40 T-Ag. a The ADD isoforms were fused to GFP and the NLS derived from SV40 T-Ag. The fusion proteins (GFP-NLSSV40, GFP-NLSSV40-ADD1, GFP-NLSSV40-ADD2, and GFP-NLSSV40-ADD3) were transiently expressed in HEK293 cells and their expression was analyzed by immunoblotting with anti-GFP antibody. b The constructs described in panel a were transiently expressed in NIH3T3 cells and their distribution in the nucleus and cytoplasm was measured (n > 600). The values (mean ± SD) are from three experiments