Fig. 1
Characterization of cultured olfactory ensheathing cells (OEC). a: low affinity nerve growth factor receptor (p75NGFR) immunoreactivity of OEC, (b): S100 immunoreactivity of OEC, (c): GFAP immunoreactivity of OEC, (d): OEC expressing GFP at 3 days after Ad-GFP infection. p75NGFR(+) cells 90.8 ± 4%; S100(+) cells, 91.3 ± 2.9%; weak GFAP (+) cells, 97.0 ± 1.3%. Nuclei are counterstained with Hoechst 33258 (blue). Bar = 50 μm. Data are expressed as average ± SD and represent the results of two independent experiments performed in triplicate (n = 6 per experimental conditions). Note that OEC are highly permissive to Ad-GFP infection