Fig. 2From: Improving the regenerative potential of olfactory ensheathing cells by overexpressing prostacyclin synthetase and its application in spinal cord repairThe enzyme activity and respective protein expression of eicosanoid biosynthesis in cultured OEC. a & b: HPLC analysis of 14C-labelled eicosanoids generated in Control and AdPGIS-transduced OEC cultures, respectively, in response to [1-14C] arachidonic acid (AA). 6-KP denotes 6-keto-PGF1α, the product of PGI2 hydrolysis. Each prostanoid peak was verified by coelution with an authentic radiolabelled prostanoid, c: Western blot analysis of protein levels of PGIS, COX-1 and COX-2 in cultured OEC receiving AdPGK or AdPGIS infection. Measurement of eicosanoid biosynthesis and biosynthetic enzyme expression in cultures was conducted at 3 days after Ads transduction. Confluent OEC cultures were pulsed with [1-14C] AA for 10 min. The released [1-14C] eicosanoids in the released medium of OEC were purified and subsequently analyzed by HPLC with a radiochemical flow-through detector. Note that OEC constitutively expressed COX-1 and COX-2 but not PGIS. On Ad-PGIS infection, PGIS expression was increased and AA metabolites in OEC were shunted through 6-ketoPGF1α (6-KP) synthesisBack to article page