Fig. 2

Effects of SNHG15 on OS cell proliferation, invasion, migration and autophagy. a qRT-PCR was performed to determine the expressions of SNHG15 in five OS cell lines (143B, U2OS, HOS, MG63 and SaOS2) and osteoblastic cell line HFOB1.19. GAPDH was used as the endogenous control. b The expression of SNHG15 in U2OS cells transfected with si-SNHG15–1, si-SNHG15–2, si-SNHG15–3 or si-control was evaluated by qRT-PCR. GAPDH was used as the endogenous control. c The expression of SNHG15 in MG63 cells transfected with pcDNA-SNHG15 or vector was assessed by qRT-PCR. GAPDH was used as the endogenous control. MTT assay was carried out to detect cell proliferation at 24 h, 48 h, 72 h, and 96 h in U2OS cells (d) transfected with si-SNHG15 or si-control and MG63 cells (e) transfected with pcDNA-SNHG15 or vector. Transwell invasion assay in U2OS cells (f) transfected with si-SNHG15 or si-control and MG63 cells (g) transfected with pcDNA-SNHG15 or vector were performed to detect cell invasiveness. Transwell migration assay in U2OS cells (h) transfected with si-SNHG15 or si-control and MG63 cells (i) transfected with pcDNA-SNHG15 or vector were performed to detect cell migration. Western blot was used to evaluate the levels of Atg5, LC3-I, LC3-II and p62 in U2OS cells (j) transfected with si-SNHG15 or si-control and MG63 cells (k) transfected with pcDNA-SNHG15 or vector. β-actin was used as the internal control. *P < 0.05 vs. control group