Fig. 5

SNHG15 contributed to proliferation, invasion, migration and autophagy by sponging miR-141 in OS cells. U2OS cells were transfected with si-SNHG15, si-control, si-SNHG15 + anti-miR-141, or si-SNHG15 + anti-miR-control. MG63 cells were transfected with pcDNA-SNHG15, vector, pcDNA-SNHG15 + miR-141, or pcDNA-SNHG15 + miR-control. Transfected U2OS and MG63 cells were maintained for 48 h. MTT assay was performed to detect cell proliferation at 24 h, 48 h and 72 h in transfected U2OS (a) and MG63 (b) cells. Transwell invasion assay was used to assess cell invasiveness in transfected U2OS (c) and MG63 (d) cells. Transwell migration assay was used to assess cell migration in transfected U2OS (e) and MG63 (f) cells. Western blot analysis was carried out to determine the levels of Atg5, LC3-I, LC3-II and p62 in transfected U2OS (g) and MG63 (h) cells. β-actin was used to normalize the levels of Atg5, LC3-I and LC3-II. *P < 0.05 vs. control group