Fig. 2
POSTN regulates the ERK/NF-κB axis to modulate ovarian cancer cell migration and invasion. A Western blot analysis for detecting phosphoproteins and proteins as indicated in SKOV3 cells transfected with POSTN or in SKOV-I6 cells infected with lentiviral vectors encoding shPOSTN or a scrambled control. B PLA images show protein–protein interactions between POSTN and integrin β3 or integrin β5 in SKOV-I6 cells. The scale bar represents 25 μm. C Western blot analysis for detecting phosphoproteins and proteins as indicated in SKOV3 cells treated with recombinant POSTN protein (100 ng/mL), in combination with anti-αvβ3 (10 μg/mL) or anti-αvβ5 (10 μg/mL) neutralizing antibody as indicated. D Western blot analysis for measuring proteins and phosphoprotein as indicated in SKOV-I6 cells transfected with siITGB5 or control oligonucleotides. E Western blot analysis for detecting p-ERK and ERK in SKOV-I6 cells treated with antibody against POSTN or IgG. F, G Migration and invasion assays of SKOV3/POSTN or SKOV-I6 cells treated with a specific POSTN monoclonal antibody (2 μg/mL) or IgG. **p < 0.01; *** p < 0.001