Fig. 2

LSD1 is induced by oxidative stress in a motor neuronal cell line (NSC-34). A A scheme illustrating a work flow of cell experiment. B Confocal microscopy shows that oxidative stress increased LSD1 immunoreactivity (red) in the nucleus of NSC-34 cells. The nucleus was counterstained with DAPI (blue). Scale bars (white): 10 μm. C Confocal microscopy shows that oxidative stress (H₂O₂) reduced H3K4me2, a substrate of LSD1, in NSC-34 cells. Scale bars (white): 10 μm. D Quantification of LSD1 and H3K4me2 immunoreactivity level. The number of cell counting: 30 cells/group. E Western blot analysis confirmed that oxidative stress increased LSD1 level but decreased H3K4me2 level in the NSC-34 motor neuron cells. F Quantification of LSD1 and H3K4m2 levels from Western blot analysis (originated from E). Significantly different at *p < 0.05, **p < 0.01, and ***p < 0.001