Fig. 8

Herboxidiene efficiently inhibits the replication of influenza A virus (IAV). A549 cells were infected at a MOI of 0.001 with H1N1 (A/WSN/33) or H3N2 (A/2032/2017) virus after 36 h of infection. a Protein expression was determined by western blotting using specific antibodies. b Total RNA was collected and identified using RT-PCR, 1F and 1R primers were used to detect different M transcripts, and 3F and 1R primers were used to determine the expression of all M transcripts. c Plaque formation was visualized through crystal violet staining