Fig. 7

TFBP mitigates TBI-mediated expression of activation of microglial cells. CCI induces morphological changes in microglial cells (A), which are representative of an activated phenotype. Multiple parameters of Iba1 + cell morphology were analyzed, including ramification index (B), spanned area (C), number of branches (D), junctions (E) and endpoints (F). TFBP (16.25 mg/kg and 32.5 mg/kg, i.p.) mitigated the morphological changes induced by CCI in cerebral cortex, evaluated at 2 weeks post-injury (B–F). Representative images of Iba1 + cells at × 40 magnification and their skeleton reconstruction through MotiQ software (A). *p < 0.05, ****p < 0.0001 vs control group; ^#p < 0.05, ^##p < 0.01, ^###p < 0.001, ^####p < 0.0001 vs CCI group; + + + + p < 0.0001 vs Contralateral side. ‘N value of animals’ shown at the base of each bar within brackets