Fig. 1

Identification and characterization of cardiac ferroptosis-associated circRNAs. a The relative circRNA expression from Sham vs. I/R-treated mice was exhibited by volcano plot. The circRNA in the upper left quadrant and upper right quadrant are significantly differentially expressed. b The expression levels of upregulated circRNAs (screened from RNA-seq data) in heart tissues with I/R injury were determined by qPCR. c, d The expression levels of downregulated circRNAs were determined by qPCR in heart tissues with I/R injury or in cardiomyocytes treated with H/R. e The expression level of FEACR in different tissues of mice. f The expression level of FEACR was determined by qPCR in cardiomyocytes and fibroblasts isolated from mice. g The level of FEACR in the cellular component of the nucleus and cytoplasm was detected by qPCR. The expression of U6 and GAPDH was used to determine the purity of cytoplasmic or nuclear fractions. b, c n = 3 mice per group; d n = 3 biological replicates; e, f n = 6 biological replicates; g n = 3 biological replicates. Data were mean ± SD, and the P-value was calculated by Student’s t-test (b–d, f) or One-way ANOVA (e). The experiment technically repeats three times