Fig. 2

FEACR attenuates ferroptosis in vivo. a The adenovirus expressing FEACR was delivered into the mice and the expression level of FEACR was measured by qPCR in vivo. b–i FEACR or negative control (NC) was overexpressed by delivery of the adenovirus expressing FEACR or NC and then performed I/R injury or sham in mice. b The midventricular heart section was stained by Evans blue-TTC and representative images are shown in upper panels as blue-healthy area, red-risk area, white-infarcted area, scale bar, 2 mm. The chart below displays infarct sizes. AAR refers to the area at risk, and INF refers to the infarct area. c The left ventricular fractional shortening (FS) was monitored by the transthoracic ultrasonic imaging system. d Ferroptosis-related proteins were examined by Western blot (upper) and were quantified (lower). e Ptgs2 level was detected by qPCR. f Representative images of Prussian blue iron staining in cardiac tissue. The black arrow indicated ferric iron. g The level of ferrous iron content in the serum. h The level of lipid peroxide MDA in each group. i Quantitative statistics of ROS content stained with C11-BODIPY in cardiomyocytes. a n = 5 mice per group; b, c n = 8 mice per group; d n = 3 biological replicates; e, h n = 5 mice per group; g, i n = 6 mice per group. Data were mean ± SD, and the P-value was calculated by One-way ANOVA. The experiment technically repeats three times