Fig. 5

NAMPT inhibits ferroptosis in vitro and in vivo. a The level of NAMPT was determined by Western blot in cardiomyocytes treated with H/R at indicated time points. b–d Cardiomyocytes were infected with adenovirus harboring NAMPT or NC and then were treated with H/R. b The cell survival rate was assayed. c The ferroptosis-related proteins were detected by immunoblot. d The level of MDA was tested. e The expression of NAMPT in cardiac tissue from myocardial I/R of mice. f–i The adenovirus harboring mouse NAMPT or NC was injected into a mouse and subjected to I/R. f Evans blue-TTC staining was applied to determine the infarcted zone of the midventricular area, scale bar, 2 mm. g FS was determined by transthoracic echocardiographic analysis. h, i The level of lipid peroxidation MDA and ptgs2 mRNA was measured in the NAMPT overexpressed myocardium upon I/R injury. a n = 3 biological replicates; b n = 6 biological replicates; c n = 3 biological replicates; d n = 5 biological replicates; e n = 3 mice; f, g n = 8 mice; h, i n = 5 mice. Data were mean ± SD, and the P-value was calculated by One-way ANOVA (a–d, f–i) or student’s t-test (e). The experiment technically repeats three times